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KMID : 0545119910010010050
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 1 p.50 ~ p.53
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Analysis of the Reaction Steps in the Bioconversion of D,L-ATC to L-Cysteine
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Ryu Ok-Hee
Shin Chul-Soo
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Abstract
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The reaction steps involved in the bioconversion of a chemically synthesized precursor, D,L-2-amino-Δ^2-thiazollne-4-carboxylic acid (D,L-ATC), to L-cysteine and the properties of the involved enzymes were investigated. It was found that the conversion consisted of two steps, i. e., D,L-ATC to S-carbamyl-L-cysteine (S-C-L-cysteine) and S-C-L-cysteine to L-cysteine, and the S-C-L-cysteine was an intermediate between them. While the enzymes involved in the reactions were induced by the addition of D,L-ATC as an inducer, S-C-L-cysteine induced only the enzyme involved in the latter step. The conversion of S-C-L-cysteine to L-cysteine could be also carried out in the presence of hydroxylamine and its rate was much faster than that by the corresponding enzyme. On the other hand, L-cysteine (or L-cystine) was decomposed to evolve H_2S by the enzyme considered to be a kind of desulfhydrase. However, hydroxylamine was a perfect inhibitor for this enzyme.
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